Coding

Part:BBa_K316003:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-22)

XylE - catechol 2,3-dioxygenase from P.putida with terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 509
    Illegal AgeI site found at 860
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The parts were put together using standard assembly [1]


Source

XylE was obtained from the registry BBa_K118021, the terminator is BBa_B0014. XylE is naturally found in Pseudomonas putida.

References